Hands-On Zebrafish Xenografts

Due to the COVID-19 Outbreak this course will be postponed (date to be determined). If you want to be informed when a new date is announced, please fill in this form.

The zebrafish, Danio rerio, has emerged as a powerful model system for the study of vertebrate development but also disease, in particular cancer.

This intensive hands-on short course will focus on the technicalities of generating zebrafish xenografts using human cancer cell lines.
It will cover:

• Protocols for preparation of human cancer cells for injection
• Generation of zebrafish xenografts (microinjection in the perivitelline space)
• Metastatic assay
• Protocols for immunofluorescence
• Mounting xenografts for confocal imaging
• Confocal session

---

Due to its strong practical component, this course has a limit of 8 participants. Applicants are requested to send a CV and motivation letter, for selection procedures.