Hands-On Zebrafish Xenografts

Due to the COVID-19 Outbreak this course will be postponed (date to be determined). If you want to be informed when a new date is announced, please fill in this form.

A course by the Fior Lab

1-5 June, 2020

The zebrafish, Danio rerio, has emerged as a powerful model system for the study of vertebrate development but also disease, in particular cancer.

This intensive hands-on short course will focus on the technicalities of generating zebrafish xenografts using human cancer cell lines.
It will cover:

  • Protocols for preparation of human cancer cells for injection
  • Generation of zebrafish xenografts (microinjection in the perivitelline space)
  • Metastatic assay
  • Protocols for immunofluorescence
  • Mounting xenografts for confocal imaging
  • Confocal session


Champalimaud Center for the Unknown, Lisbon


Deadline: March 30;

Confirmation: April 10


Academia: 1000€;

Industry: 2000€

Course materials; lunch and coffee-breaks; and closing dinner included.

Due to its strong practical component, this course has a limit of 8 participants. Applicants are requested to send a CV and motivation letter, for selection procedures.


Scientific Coordination – Rita Fior


Marta Estrada
Bruna Costa
Vanda Póvoa
Cátia Almeida
Mayra Martinez-Lopez

Organizing Committee

Rita Fior
Catarina Certal
João Cruz

Additional information



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