Hands-On Zebrafish Xenografts


A course by the Fior Lab

2-6 May, 2022


The zebrafish, Danio rerio, has emerged as a powerful model system for the study of vertebrate development but also disease, in particular cancer.

This intensive hands-on short course will focus on the technicalities of generating zebrafish xenografts using human cancer cell lines.
It will cover:

  • Protocols for preparation of human cancer cells for injection
  • Generation of zebrafish xenografts (microinjection in the perivitelline space)
  • Metastatic assay
  • Protocols for immunofluorescence
  • Mounting xenografts for confocal imaging
  • Confocal session


Where:

Champalimaud Center for the Unknown, Lisbon

Application:

Deadline: February 28;

Confirmation: March 10

Fees:

Academia: 1500€;

Industry: 2500€

Course materials; lunch and coffee-breaks; and closing dinner included.

Due to its strong practical component, this course has a limit of 8 participants. Applicants are requested to send a CV and motivation letter, for selection procedures.
Selected participants will have to present a valid digital COVID certification.

Organization

Scientific Coordination – Rita Fior

Faculty

Rita Fior
Marta Estrada
Raquel Mendes
Vanda Póvoa
Cátia Almeida
Márcia Fontes

Organizing Committee

Rita Fior
Catarina Certal
João Cruz

Additional information

xenografts@congento.org


Sponsors


If you would like to sponsor this workshop, please contact us at xenografts@congento.org






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