Molecular Biology and Virus Production

Molecular Biology Services
  • Production of Chemical and Electrocompetent Bacterial Cells
  • DNA amplification and isolation
  • Genomic DNA extraction/Total RNA extraction
  • Analysis of RNA/DNA integrity (BioAnalyzer 2100, Agilent)
  • cDNA production (Reverse Transcription)
  • ISH probe cloning and production (Fluo/Dig)
  • Custom made Molecular Cloning:
– Restriction Enzyme Cloning
– Gateway® Recombination Cloning
– TOPO® Cloning (Blunt/TA)
– Isothermal Assembly Reaction (Gibson Assembly)
  • Site-Directed Mutagenesis (substitutions/deletions/insertions)
  • BAC Transgenesis
  • Design of primers/ gRNA for transgenic approaches
  • In-vitro transcription
  • PCR/RT-PCR
  • Mycoplasma Testing of Cell Cultures
  • Recombinant proteins
Virus Production Services
  • Adeno associated virus (AAVs)
  • Adenovirus
  • Lentivirus
  • Retrovirus
  • Self-inactivating rabies virus (SiR)

Consulting and training

contact: molecular@congento.org

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