Molecular Biology and Virus Production

Being a cross-cutting service to all the model organisms under the scope of the infrastructure, and gathering a diverse scientific know-how and highly-skilled technicians, CONGENTO is able to offer a variety of services regarding Molecular Biology and Virus Production.
Aware of the growing importance of non-animal methods, and in line with the 3R policy, CONGENTO also presents a service dedicated to CRISPR/Cas editing for cell lines.

  • Molecular Biology
    • Production of Chemical and Electrocompetent Bacterial Cells
    • DNA amplification and isolation
    • Genomic DNA extraction/Total RNA extraction
    • Analysis of RNA/DNA integrity (BioAnalyzer 2100, Agilent)
    • cDNA production (Reverse Transcription)
    • ISH probe cloning and production (Fluo/Dig)
    • Custom made Molecular Cloning:
– Restriction Enzyme Cloning
– Gateway® Recombination Cloning
– TOPO® Cloning (Blunt/TA)
– Isothermal Assembly Reaction (Gibson Assembly)
    • Site-Directed Mutagenesis (substitutions/deletions/insertions)
    • BAC Transgenesis
    • Design of primers/ gRNA for transgenic approaches
    • Full Transgenic Animal Projects
    • In-vitro transcription
    • PCR/RT-PCR
    • Cell Culture projects
    • Mycoplasma Testing of Cell Cultures
    • Recombinant proteins
  • CRISPR in Cell Lines – get more details here
  • Virus Production
    • Adeno associated virus (AAVs)
    • Adenovirus
    • Lentivirus
    • Retrovirus
    • Self-inactivating rabies virus (SiR)
  • Consulting and training

For any inquiries regarding CONGENTO Molecular Biology and Virus Production services, please fill the form below or e-mail

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