Being a cross-cutting service to all the model organisms under the scope of the infrastructure, and gathering a diverse scientific know-how and highly-skilled technicians, CONGENTO is able to offer a variety of services regarding Molecular Biology and Virus Production.
Aware of the growing importance of non-animal methods, and in line with the 3R policy, CONGENTO also presents a service dedicated to CRISPR/Cas editing for cell lines.
- Molecular Biology
- Production of Chemical and Electrocompetent Bacterial Cells
- DNA amplification and isolation
- Genomic DNA extraction/Total RNA extraction
- Analysis of RNA/DNA integrity (BioAnalyzer 2100, Agilent)
- cDNA production (Reverse Transcription)
- ISH probe cloning and production (Fluo/Dig)
- Custom made Molecular Cloning:
– Restriction Enzyme Cloning
– Gateway® Recombination Cloning
– TOPO® Cloning (Blunt/TA)
– Isothermal Assembly Reaction (Gibson Assembly)
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- Site-Directed Mutagenesis (substitutions/deletions/insertions)
- BAC Transgenesis
- Design of primers/ gRNA for transgenic approaches
- Full Transgenic Animal Projects
- In-vitro transcription
- PCR/RT-PCR
- Cell Culture projects
- Mycoplasma Testing of Cell Cultures
- Recombinant proteins
- CRISPR in Cell Lines – get more details here
- Virus Production
- Adeno associated virus (AAVs)
- Adenovirus
- Lentivirus
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Retrovirus
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Self-inactivating rabies virus (SiR)
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Consulting and training
For any inquiries regarding CONGENTO Molecular Biology and Virus Production services, please fill the form below or e-mail molecular@congento.org